Author : Mahitha Banala, Samatha Talari, Archana Pamulaparthi, Shama Nazrin, Ramaswamy Na [ Volume No.:VII, Issue No.IV-July 2018] [Page No : 870-875] [2018]

An efficient protocol for multiple shoot induction and plantlet establishment was achieved through immature seed culture in Cajanus cajan cv ICP 26. Immature seeds of C. cajan were inoculated on MS/B5 medium containing 10-30 gm/L of sucrose and also on MS medium supplemented with various concentrations of BAP (0.5-5.0 mg/L). Absolute percentage (100%) of seed germination was observed on MS medium supplemented with 25/30 gm/L sucrose. The maximum frequency of multiple shoot induction was observed on MS medium fortified with 2.0 mg/L BAP. The proliferated multiple shoots were elongated at 0.5 mg/L GA3 in combination with 2.0 mg/L BAP. Profuse rhizogenesis was observed on MS medium fortified with 1.0 mg/L IBA. In vitro raised plantlets were acclimatized and successfully transplanted into the field with a survival rate of 70%. Regenerated plantlets are similar to donar plants. We have established the reproducible regeneration protocol in pigeon pea cv. ICP 26 through immature seed culture which can be used for in planta transformation to introduce gene(s) of interest in pigeon pea.